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Our study globally characterized the Spx regulatory network, revealing its role in the basal expression of some In vitro transcription confirmed activation by oxidized Spx of seven newly identified promoters, of which one was also activated by Strongly correlated with observed activation. Spx-controlled promoters exhibited an extended −35 box in which nucleotide composition at the −43/−44 positions In wild-type and Δspx strains exposed or not to diamide revealed that 144 transcription units comprising 275 genes were potentially under direct Correlation of Spx–RNAP-binding sites with gene differential expression In parallel with the number and occupancy of DNA sites. Were located near Sigma(A)-dependent promoters, and upon diamide treatment, the fraction of the Spx–RNAP complex increased Here we identified 283 discrete chromosomal sites potentiallyīound by the Spx–RNA polymerase (Spx–RNAP) complex using chromatin immunoprecipitation of Spx. 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